Composite

Part:BBa_K5115052:Design

Designed by: Yi Shi   Group: iGEM24_Fudan   (2024-09-19)


ribozyme connected hox and hyp, without hoxF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 485
    Illegal BglII site found at 563
    Illegal BamHI site found at 956
    Illegal XhoI site found at 493
    Illegal XhoI site found at 685
    Illegal XhoI site found at 1163
    Illegal XhoI site found at 3402
    Illegal XhoI site found at 5231
    Illegal XhoI site found at 6393
    Illegal XhoI site found at 8232
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1256
    Illegal NgoMIV site found at 1936
    Illegal NgoMIV site found at 4543
    Illegal NgoMIV site found at 4650
    Illegal NgoMIV site found at 4920
    Illegal NgoMIV site found at 5766
    Illegal NgoMIV site found at 5998
    Illegal AgeI site found at 446
    Illegal AgeI site found at 5718
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 267
    Illegal BsaI site found at 429
    Illegal BsaI site found at 3056
    Illegal SapI.rc site found at 378


Design Notes

none


Source

synthetic

References